Schmidt and Capecchi found out by designing mice with the core of the TBP sequence intact, but a radically altered N-terminus.
In case p-18, a p-AML with a t(8 21), a 1Mb loss on 8q21.3 amputated the first 5' exons of RUNX1T1, probably inactivating the ETO-AML1 chimeric gene on the der(21) and left the 3' RUNX1T1 coding sequence intact in the AML1-ETO transcript on the der(8) [22].
The recombinant T. maritima tRNase ZS cleaves after the CCA sequence which is encoded by all but one tRNA gene, leaving the CCA sequence intact [ 30].
These high frequency mutation regions could be bracketing DNA fragments and assisting in their horizontal movement, while keeping the intervening sequence intact.
Alternatively genetic mouse models could be generated which would knock-out the putative matreshka while leaving the parent RefSeq sequence intact, thus enabling phenotypic analysis.
Nonetheless our der(14) conformed in structure to the predominant der(14) model from the database, whereby the IGHJ locus adjoins BCL-2, coding sequence intact.
Read pairs that map well with the Alu sequence intact represent AluYb8/9 insertions that are present in the reference genome.
RAM and RNMT cDNAs were used which were resistant to siRNAs due to mutation of the wobble codon (which left the amino acid sequence intact).
While its open-reading frame contains a Kozak translational initiation sequence, intact endogenous ANP32C protein has not yet been reported in biochemical isolations.
We then produced the complete set of DNA sequences, which differ from the original by one nucleotide, by mutating all individual positions but keeping the rest of the sequence intact.
Taken together, this suggests some selection pressure to conserve the C-terminal part of the Cx43 amino acid sequence intact in order to avoid impaired regulation of gap junction communication.
