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The algorithm described in the Methods Section enables analysis of the aligned reads and the identification of sequence incongruities between the predicted gene models and the observed transcriptomic data.
The algorithm described in the Methods Section enables analysis of the aligned reads and the identification of sequence incongruities between the predicted gene models and the observed transcriptomic data (Figure 1, B).
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Manual controls between ab initio predictions and sequences alignments suggest numerous incongruities.
Illumina contigs were aligned against the PacBio consensus sequence using BLAST and 187 incongruities in the Illumina data were identified.
In this study, we applied a uniform method including sequence similarity, homolog distribution, phylogenetic incongruity and other information to detect HGT events between bacteria and the five insects.
Several phylogenetic incongruities supported by pairwise sequence comparisons and synonymous distance estimates provide evidence for HT of CR elements both within and between members of the oryzoid and panicoid lineages.
To obtain a more accurate estimate for the total number of transfer events, we assigned multiple ORFs from different gene families to the same HGT event if they were adjacent in a chromosome sequence and shared the same phylogenetic incongruities as determined from embedded quartets.
There was no significant evidence of signal incongruity in the mtSSU sequence relative to the EF-1α, NIR, and PP sequences, and hence a parsimony optimized topology was derived from the unmodified alignment.
This crudely anti-Russian (Crimean War-era) chronicle used a hodgepodge of picturesque and absurd effects arranged casually or with deliberate incongruity into a loose chronological sequence.
Following BLAST, both Illumina and PacBio reads were aligned to the chloroplast reference sequence using SMALT and BLAT respectively and the incongruities were resolved by taking the majority-rules nucleotide from the two alignments at these sites.
Tapparel et al. (2009) observed phylogenetic incongruities in 5' UTR, VP1 and 3CD sequences of two clinical isolates of HRV-A related to recombination [38].
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