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Researchers in a few years will have trouble imagining how we studied human biology without the genome sequence in front of us.
It had this thundery Bernard Herrmann score, and when Robert De Niro did his "are you talking to me?" sequence in front of the mirror you suddenly sensed the degree of anger there.
The Epsom winner is generally the 11-10 favourito to continue the Ballydoyle sequence, in front of Libertarian at around 7-2, wiTradinging Leather, Sugar Boy and Galileo Rock vying for third spot.
The P-plasmid was constructed by cloning the 2 kb sequence in front of the first exon of Olig2 (mm8_chr16:91,112,391 — 91,114,400) upstream of the GFP gene on the pEGFP plasmid.
To further understand the role that the sequence in front of the armadillo repeats within APC222 653 plays in Cdc42 interaction, we made two shorter constructs: one without the armadillo repeats (APC222 453) and the other with only the armadillo repeats of APC222 653 (APC453–653), both tagged with mRFP.
A BamHI site is present 3' of the E-tag sequence in front of the stop codon when no tag is to be attached.
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Inclusion of two successive terminator sequences in front of the I-SceI cleavage site obviated this problem and resulted in highly reliable chromosomal manipulation.
No enrichment was found for the putative PdhR target sequences in front of the genes metB (factor 0.98) and ynfM (factor 0.06).
It is approximately 800 residues longer than other MAGE proteins due to the insertion of a large number of short repetitive sequences in front of the MAGE-homologous sequence.
The pCiTH-WGA, pCiTH-Kaede, pCi-vGluT-Kaede, pCivGAT-Kaede and pCi-VachT-Kaede were made inserting the 5' upstream corresponding sequences in front of reporter genes, either Kaede or Wheat germ agglutinin (WGA) as described in [ 36, 37].
The c-Myc responsive luciferase reporter plasmid, pMyc-TA-Luc (Clontech, Mountain View, CA), carries six c-Myc binding sequences in front of the minimal TATA box from the herpes simplex thymidine kinase (HSV-TK) promoter.
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