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In the intervening years the human genome sequence has been refined, with the most recent assembly being Build 37.2 (Greleased) released by the Genome Reference Consortium in 2010 [ 7].
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The design of bioreactors to facilitate this sequence of events has been refined to the extent that some bioartificial organs grown in these systems have been transplanted into recipient animals with sustained, though limited, function.
The predictive power of these models has been refined from sequence analyses of NRPS A-domains with known specificity combined with examples of crystal structures of A-domains [ 9- 11], thus identifying amino acid residues crucial for A-domain specificity.
This technique was first described by Sanger et al. [ 7] and has been refined and used as the basis of genome sequencing and assembly ever since.
With more sequence data available, phylogenetic relationships became clearer, and the model of macaque KIR genetics has been refined.
Virtuosity has been refined into concentration.
The iPod Nano has been refined, too.
This rationale has been refined over time.
Most of my craft has been refined for millennia.
Since January the plan has been refined.
Of course, 11 years later the formula has been refined.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com