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Also the sequence has been changed.
Each oligonucleotide probe is paired with a mismatch probe in which the central base in the sequence has been changed.
The C2-motif is also present in the C-terminus of MdMYB3, but its first amino acid sequence has been changed from Phe to Ser.
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Second, the IRES sequence had been changed to restore a wild type sequence, which enhances the translation of eGFP about 10 fold without affecting any other important parameters (e.g. viral titer or expression of the 5' cDNA).
However, it is difficult to infer more information on recent H. pylori evolution from the H. acinonychis mutations because the sequences have been changed to a great extent.
The fact that the sequences have been changed is indicative of a higher quality sequence.
These enzymes might be non-homologous but evolved into the same function, or the functions have been maintained although their sequences have been changed.
As visualized in Fig. 2E (bottom panel) the cDNA coding sequence of the CSB wt expression vector has been changed at nucleotide position 3249 (A to G), 3255 (T to G) and 3262 (A to G). Cell viability was evaluated using MTT [3- 4,5-dimethylthiazol-2-yl -2,5-diphenyl-2H-tetrazolium bromide] cell proliferation assay.
This has been changed.
Something has been changed.
Nothing has been changed.
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