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The need for an accessible 5′-end of CpG DNA for activity suggested that the receptor reads the DNA sequence from this end.
To evaluate how the assembled genome sequence from this project compares to the N. punctiforme genome released by the JGI in 2014 (GenBank ID: CP001037.1; not published), we aligned both assembled genomes to each other.
The 480 bp DNA barcode sequence from this specimen is GenBank HQ024540.
We used the DNA sequence from this gene to search the various nucleotide databases for similar predicted proteins.
We PCR amplified the mRelB sequence from this plasmid and inserted it into the pcDNA3.1-Myc/HisB vector (Invitrogen, Carlsbad, CA, USA).
A 917 bp sequence from this fragment was aligned between all individuals spanning 649 bp from intron 19, the entire exon 20 containing the 1014 codon (210 bp) and 59 bp in intron 20.
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All sequences from this fully-sequenced group could therefore be directly selected for further full-length analysis.
Moreover, a new conotoxin, never described to date, was also captured, identified and sequenced from this venom.
Sequences from this study are shown in bold and described as clone name (accession number) T-RF size.
Sequences from this study are shown in bold and are described as clone name (accession number) T-RF size.
Denisovans were discovered when their genome was sequenced from this pinky finger bone from a young girl.
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