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It was observed that there is no particular sequence for water absorption behavior and 2.5 kGy samples showed the lowest water uptake followed by 1 kGy irradiated sample.
Standard monodimensional spectra were acquired using a selective presaturation pulse sequence for water signal suppression as described elsewhere [24].
The proton spectra were acquired at 500 MHz on a Bruker Advance III spectrometer, with the noesy1D sequence for water suppression.
For structural determination, phase-sensitive 2D H-H NOESY, H-H COSY, and H-H TOCSY data sets (MLEV17) were recorded at 293 K with a presaturation or with a 3 9 19 pulse sequence for water suppression.
Spectra were acquired at 300 K using the standard 1D-NOESY pulse sequence for water suppression with a mixing time of 1 ms and a recycle time of 21.5 s.
A standard Bruker "ZGPR" pulse program that applies a presaturation pulse sequence for water suppression was used, and a total of 64 scans were collected into 32 K data points with a relaxation delay of 2 sec.
Similar(53)
NOESY spectra for observation of exchangeable protons were recorded in 9 1 H2O:D2O (v/v), using the Watergate pulse sequence (34) for water suppression.
In addition, large numbers of reads in the sequencing data for water buffalo, bighorn sheep and Dall sheep mapped to BoERV15 and BoERV29 (Table 2).
The genome of domestic sheep (Ovis aries, Oar_v3.1) [ 12] and contigs from the whole-genome sequencing projects for water buffalo (Bubalus bubalis, Bubalus_bubalis_Jaffrabadi_v2.0, GenBank: ACZF000000000) and goats (Capra hircus, GenBank: AJPT00000000) [ 11] were retrieved from the NCBI server (Table 4).
The sequence of water entering points differed for each of the 4 trials each day, but the location of the platform remained constant throughout the study.
CHESS sequence was used for water suppression.
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