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The raw sequence files are available on the NCBI database.
ClassyFire accepts different types of chemical input: SMILES, SDF, InChI, IUPAC name, and FASTA sequence files.
Plasmid sequence files with annotations were obtained from NCBI.
Multiple sequence files can be loaded and analysed together.
Overlapping sequence files between two 1-Mb files were inputted manually when necessary.
Sequence files were visualised using the Chromas 2.01 software (Technelysium Pty Ltd).
The standard software GS20 package was used to generate the sequence files.
Continuous images were taken at 10 s intervals and stored as TIFF image sequence files.
Sequence files were analyzed using the SAGEparser program [ 83].
Raw sequence files were trimmed and aligned using Bioedit 7.0.9.
Custom Perl scripts were used for manipulating sequence files.
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