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In all, 94 randomly picked clones were characterized with respect to sequence, expression level, and catalytic activity in the conjugation reactions between glutathione and six alternative electrophilic substrates.
Although the protein sequence of the γ2 subunit has previously been published, the cDNA sequence, expression pattern, genomic structure, and localisation of the human GNG2 gene have not been described.
Intronic sequence expression between exon 1 and exon 2 may impact on the function of CD74, because it is located within the trimerisation domain of the protein.
Elucidation of the sequence, expression, and regulatory role of each of these transcripts should shed light on the P. aeruginosa-specific arrangement of the prrF genes.
Due to the similarities in sequence, expression patterns and antimicrobial activity, feCath belongs to the linear α-helix subgroup of cathelicidins found in dogs, mice, rats and humans.
Aerts et al. (2006) developed Endeavour, a gene prioritization method that ranks candidate genes based on their similarity to genes already involved in the biological process of interest, using multiple data sources (e.g., sequence, expression, literature) [2].
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Two examples are provided where protein sequence expressions are presented for two patients (see Fig. 3).
Among them, miR156d*, miR157b*, and miR3630* star sequence expressions were rather low.
This removes the uncertainties in the repeat lengths of (A), (C), (G) and (T) in the sequence expressions above.
Finally, the chapter concludes with a brief discussion of the future of genetic association studies, including newer biologic methods/targets (including sequencing, expression, copy number variation, and epigenetics), and analytic strategies (gene/environment interaction and pathway analysis).
Both of these short runs had low or essentially no identity with any yet sequenced expression cassette.
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