Before scanning, subjects were required to perform the complex sequence, error-free, eight times from memory.
For several specific sequences, error-free decoding can be achieved by a few iterations.
In a hypothetical ideal case where the target sequence matches exactly the reference sequence, and read sequencing is error free, we will not have any misaligned reads; Pmis=0, and Puq is maximized (multiple matches are only resulted from reference similarities).
Adding to this uncertainty, the analytical processes involved in massively parallel sequencing/whole-genome sequencing are not error free.
If the coverage is large enough and the sequenced reads are error free, there should be only one contig containing the entire genome.
In another instance, Young and colleagues combined dual asymmetrical PCR and overlap extension PCR, which enables any DNA sequence to be synthesized error free.
However, this position can be corrected by manual editing with high safety through existing error free forward sequence reads.
Filtered reads is the number of sequence reads after each sequence was trimmed to 70 bp; sequences with less than 80% chance of being error free were discarded from further analysis.
Given the high sequencing coverage underlying the consensus sequence, we are fairly confident that it is error free.
Sequences obtained from the HiSeq2000 were trimmed to 70 bp by removing the last 30 bp (to match the GAII sequences), and any reads with less than 80% chance of being error free were discarded.
In summary, we are confident that the entire nucleotide sequence of each construct posted on the web site [ 23] is error free.
