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The complete catalytic machinery (catalytic triad and two oxyanion hole residues) could be annotated in 91% of LED sequence entries.
Based on an in silico restriction analysis of database sequence entries, suitable restriction enzymes for a T-RFLP approach were selected.
Once the populations of siRNAs generated were found to reduce the virus titre significantly, two highly conserved 21 bp siRNA duplexes were designed by analysing all FMDV sequence entries available in public-domain databases.
This resulted in 38,849 3'-UTR sequence entries.
This step helped to rescue some rejected clones which were found to be ultimately acceptable due to updated MGC sequence entries.
For example, in 1986 the Swiss-Prot [4] database contained only 3,939 protein sequence entries, but now the number has jumped to 521,016 according to the release 2010_10 on 05-Oct-2010 by the UniProtKB/Swiss-Prot at http://www.expasy.org/sprot/relnotes/relstat.html; meaning that the number of protein sequence entries now is more than 132 times the number from about 24 years ago.
All sequence entries were indeed assigned the e.22.1 FSF.
Note that mRNA sequence entries assigned to hypothetical genes were not included, and that multiple sequence entries were found for some genes.
Theoretical molecular weight and p I of characterized proteins were calculated by processing sequence entries at.
The SCOP sequence entries were checked against the UniProt annotation file for the FT_SIGNAL keywords.
The obtained cDNA sequences were sequenced and compared to the above given Genbank sequence entries.
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CEO of Professional Science Editing for Scientists @ prosciediting.com