Sentence examples for sequence engineering from inspiring English sources

Here, we modify these biomacromolecules via structural and sequence engineering coupled with selective enzymatic digestion to generate an open-ended p-shRNA (op-shRNA) that is cleaved over ten times more efficiently to yield siRNA.

We determine translation rates of messages by pulse-chase analyses in living Escherichia coli cells and show that sequence engineering based on these concepts predictably modulates translation rates in a manner that is superior to codon usage frequency, which occur during the elongation phase, and significantly impacts folding of the encoded polypeptide.

In this study, we combine multiple strategies: a) the use of aptamer probes, short oligonucleotides strands selected in order to present strong selectivity for the small molecule target, b) the sequence engineering of split versions of the aptamer for a sandwich assay and c) the amplification of Surface Plasmon Resonance imaging (SPRi) signal by the use of gold nanoparticles (AuNPs).

Finally, structure-based methods for sequence engineering [31], [32] may be implemented into this approach to identify replaceable positions and to limit libraries to manageable ensembles, which would allow further optimization of protein stability.

However, we anticipate that the design parameters for improved protein production through synonymous gene sequence engineering of open reading frames will ultimately require a more sophisticated user driven approach.

The prospect of increasingly affordable synthetic genes opens the opportunity to explore the effects of synonymous gene sequence engineering for improved protein production, or in some cases reduced protein production [ 39].

Propeptide-sequence (Pro-sequence) engineering involving site-directed mutagenesis, truncating the pro-sequence or swapping pro-sequence, were successfully used to produce recombinant keratinolytic proteases (Li et al. 2013; Rajput et al. 2012; Sharma et al. 2011).

SLIC cloning enables precise and sequence independent engineering and is based on joining vector and insert with 15 25 bp homologies on both DNA ends by homologous recombination.

Recent experimental advances in sequencing, genetic engineering and bionanotechnology will make this engineered circuit implementation feasible in near future [ 7, 36- 41].

For example, he said that sequencing and engineering processes that might yield a cell better equipped to fight disease could also yield an organism that creates ethanol from plant refuse more efficiently.

Microbes can now be designed and engineered for a particular biosynthetic purpose, thanks to recent developments in genome sequencing, metabolic engineering, and synthetic biology.