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A sequence encoding for c-myc epitope tag (c-myc; NEQKLISEEDLQC) was inserted at the C-terminus of the gene encoding the scFv to obtain NAga2p-HA- G4S 3-VL-linker-VH-c-mycC fusioNAga2p-HA- G4S 3-VL-linker-VH-c-mycC
A sequence encoding for Gly Gly dipeptide spacer (G2, NGGC) followed by a 15 amino-acid peptide sequence (AviTag) containing a defined lysine for site-specific biotinylation (NGLNDIFEAQKIEWHEC) were inserted at the C-terminus of the ELR+ CXC chemokine to obtain NCXCL-G2-AviTagC synthetic genes.
A sequence encoding for a five amino-acid flexible linker (NGGGGSC) followed by a hexa-histidine tag (His6; NHHHHHHC) was inserted at the C-terminus of the gene encoding the scFv to obtain the final NSA- G4S 3-scFv-G4S-His6C NSA- G4S 3-scFv-G4S-His6C NSA- G4S 3-scFv-G4S-His6C NSA- G4S 3-scFv-G4S-His6C NSA- G4S 3-scFv-G4S-His6C
Therefore we compared fMRI-activation in experts with those of naïve participants during task performance in the field of expertise (finger sequence encoding for pianists, creative writing for writers and portrait drawing for students of fine arts).
In comparison with commonly used plasmids, this vector is designed to have relatively low expression levels of cloned scFv antibodies due to the amber codon positioned in a sequence encoding for the PhoA leader peptide.
A nucleotide sequence encoding for the signal peptide of VSV-G (MKCLLYLAFLFIGVNC) was included upstream of each prME gene.
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The full-length AsNLRC3 transcript composed of a 4142 bp nucleic acid sequence encode for a protein of 1134 deduced amino acids.
The resulting sequence encodes for a protein of 112 amino acids with a predicted molecular mass of 12.5 kDa and an isolectric point of 5.937, and a hydrophobic character (39% of the amino acids).
The cloned HlbHLH2 cDNA sequence encoded for an acidic (pI 5.1) protein having a molecular weight of 77.1 kDa.
The 5' portion of the sequence encodes the LKR activity of the bifunctional enzyme, which is encoded by eleven exons (blue boxes in Figure 1B); the 3' part of the sequence encodes for SDH activity and contains twelve exons (red boxes).
52.46%-53.03%, 1.83%-1.86%, and 5.76%-5.84%-5.84%e genome sequence encodes for proteins, tRNAs, and rRNAs, respectively, whereas the remaining 39.31%-39.8439.31%-39.84%ing regions including intregionsntergenincludings, and pseudogenes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com