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To generate features that are discriminative of the sequence direction, we model the peptide as a directed graph.
The best-aligning results were used to determine the sequence direction of the unigenes.
The best alignment results were used to determine the sequence direction of unigenes.
The best hits were used to decide on sequence direction of transcripts.
For unigenes with sequence direction, sequences from the 5' end to the 3' end were provided.
For those without sequence direction, we obtained sequences from assembly software.
When a unigene could not be aligned, sequence direction would be confirmed using ESTscan program.
Alternative strategy could rely on reversing of sequence direction in one helix of the parallel pair.
The best matched hits were used to determine the sequence direction of each unigene.
Unigenes without an annotation were analyzed by ESTScan to predict coding regions and sequence direction.
The best alignment results were used to decide the sequence direction of unigenes.
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