The Bourne Identity was followed by a 2004 sequel, The Bourne Supremacy, which received a similar positive critical and public reception, but received some criticism for its hand-held camerawork, which observers argued made action sequences difficult to see.
This makes distantly homologous sequences difficult to distinguish from non-homologous sequences of similar amino acid content.
In these two plastid-targeted genes the different sequences are heterogeneous at the amino acid level in the signal peptide and chloroplast targeting sequence, and the length variation in this region made direct sequencing difficult.
Subtelomeric regions contain repetitive sequences or duplicated DNA fragments, which make cloning and sequencing difficult.
However, the short read length, higher complexity, and inherent incompleteness make metagenomic sequences difficult to assemble and annotate [18].
Moreover, typical errors in draft genome sequences include misassemblies of repeated sequences, collapses of repeated regions, making such sequences difficult to use for repeat content analysis.
Because adding or deleting 10 bases will shift the homologous sequences by 10, bases will be out of alignment downstream of the shift, making sequencing difficult.
However, several drawbacks have been reported in DNA sequencing, including its interpretation errors [ 14] and difficulty of high-throughput data generation, which makes DNA sequencing difficult to use.
Unlike rice, maize has a very large genome, with extensive regions of genomic repeats that make sequencing difficult (SanMiguel et al., 1996).
Additionally the small fraction of protein-coding genes that comprise eukaryotic genomes (< 25% in worms and < 5% in humans) make the identification of coding sequences against the ubiquitous background of non-coding sequences difficult [ 36].
