Exact(11)
Bioinformatics is fed by high-throughput data-generating experiments, including genomic sequence determinations and measurements of gene expression patterns.
This procedure guaranteed that the small RNAs within the reverse transcriptase could not be counted in the RNA samples during the ensuing sequence determinations.
The Sanger mtDNA genome sequence derived from the CPC98 sample was, therefore, verified through independent extractions, amplifications and sequence determinations.
After HPLC purification in conditions similar to those described in the "Table 1" legend, the obtained peptides were subjected to amino acid composition and sequence determinations.
In some cases, the NRL group outsourced (Macrogen USA, Gaithersburg, MD) corroborative de novo gene sequence determinations from amplified products from hemagglutinin (HA), matrix (M), neuraminidase (NA), non-structural (NS1) and RNA-dependent RNA polymerase subunit (PB2) genes.
The consistent results of repeated RT-PCRs and sequence determinations indicate that the polymorphism is not related to the in vitro amplifications and sequence determinations, but that the original samples contain mixtures of ABV sequences.
Similar(49)
Insert DNA was characterised by sequence determination (ABI 3700 DNA sequencer) and subsequent comparison to the public database (BLASTn, BLASTx).
19 Amplicons were then subjected to nucleotide sequence determination and analyzed on ABI 3730XL sequencer.
The nucleotide sequence determination was carried out by cycle sequencing using an ABI 3730xl DNA sequencer (GATC Biotech AG, Konstanz, Germany).
Identifying top and bottom is clearly important in sequence determination, so important in fact that a considerable literature has been devoted to this question alone.
The strict CARA sequence considers the channel during sequence determination, only.
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