b, Mapping coverage of the 3,010 rice genomes to the Nipponbare RefSeq as a function of sequence depth.
The curves show a similar level of saturation, with the automated libraries having slightly more unique molecules at the analysed sequence depth.
The 454 system generates long sequences but suffers from low sequence depth.
As expected, the frequency of Mendelian errors diminishes as a function of increased sequence depth.
Reads that were present more than 5× the estimated sequence depth were removed from the dataset.
The transcriptomic sequence depth of 23 provides a high level of confidence for the quality of these models.
By requiring a minimum sequence depth of 20 reads per sample, 94% of the successfully amplified samples were genotyped.
Primer trimming leads to a diminished sequence depth in the trimmed regions causing a diminished sequence quality.
Samples were examined for sequence depth.
Numbers between brackets represent the sequence depth.
The average sequence depth was 52, 196 and 69.
