Sentence examples for sequence correctness from inspiring English sources
Reference genes underwent the same manual checks for sequence correctness by alignment to the reference genome as above (see Additional file 8).
The top 100 genes/probe sets were also manually checked for sequence correctness by alignment to the reference genome (see Additional file 6).
Before testing our method by comparison with the large-scale data from Pal et al., we wanted to test the efficacy of our method in recovering the marginal probabilities as compared to an exhaustive enumeration of the exact sequence space, assuming correctness of the paradigmatic model.
All clones were sequenced to ensure correctness.
Whole inserts in mutated clones were sequenced to ensure correctness.
The final construct was sequenced to verify correctness of the inserted PCR fragment and transformed into Halobacterium sp. NRC-1Δ usingusing the PEG-EDTA method [ 37]. pBB400Δ gvpC transformants were selected by plating on CM+ media lacking uracil (HURA), colonies picked and grown in liquid HURA media, and genomic DNA extracted, as previously described [ 26, 37].
The orientation, frame, and correctness of sequence of each insert DNA was confirmed by restriction endonuclease analysis and automated DNA sequencing.
All RT-PCRs with the co-transformants yielded cDNA fragments with the expected sizes, and DNA sequence analysis demonstrated the correctness of the sequences.
The PCR products were also sequenced to verify the correctness of the amplification region.
All resulting vectors were sequenced to confirm the correctness of the cloned DNA fragments (SCF, CeBiTec, Bielefeld, Germany).
In this study we analyzed the transcriptome of F. graminearum grown in liquid CM medium by Illumina sequencing to investigate the correctness of predicted gene models present in the annotated Broad F. graminearum genome database and to identify the occurrence of alternative splicing, RNA editing, non-canonical splice sites, novel transcripts and the sequences of the 5′ UTR and 3′ UTR regions.
