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Sequencing was performed at the sequence core of the Leiden Genome Technology Center.
The TC score ranges from 0.0 (no core columns in the reference sequences correctly aligned) to 1.0 (all reference sequence core columns correctly aligned).
The assay involves the use of one TE-specific primer and another primer complimentary to the adaptor sequence core of the HpaII/ MspI site.
The minimum T cell stimulatory sequence (core epitope) within each selected 20-mer was determined by testing the proliferation of reactive TCL from different subjects to truncated peptide sets (e.g. Fig. 2 and Table 3).
The 6 patterns provide a sequence core to which other sequences have shown significant similarity hits in the following performed comparisons: 1) between sequences within families, and 2) between family consensus sequences and/or HMMs.
They generally consist of a core DNA sequence (core promoter) along with other regulatory elements such as enhancers, silencers and other DNA sequences, which interact with DNA binding proteins (transcription factors) to drive transgene expression in various plant cells.
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The placental transcriptomes were sequenced at the Cincinnati Children's Hospital Medical Center sequencing core, using Illumina sequencer and retrieving 30 million of 50 bp paired-end reads.
Both strands of the amplified fragments were directly cycle-sequenced, using the PCR primers, by the University of Michigan's Sequencing Core Facility.
The T/H uncertainty evaluation process performed by Westinghouse Electric Company WECC) identified the low-margin sequences (core uncovery) by expanding PRA Event Trees (ETs).
The plasmid inserts were verified by sequencing (Sequencing Core Facility CeBiTec, Bielefeld, Germany), and the plasmids were used to transform C. glutamicum ATCC 13032.
We thank for the sequencing core in NIEHS.
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