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Two others (L-CT and L-CTx3) have the sequence attached at the C-terminus of the immunoglobulin light chain with minimal conformational constraints.
The enzyme β4Gal-T7 transfers galactose to xylose during the synthesis of the tetrasaccharide linker sequence attached to a Ser residue of proteoglycans.
This UPS reporter contains a degron sequence attached to the C-terminus of green fluorescent protein, and is predominantly expressed in neurons throughout the brain of our transgenic model.
The four fusions covered a broad range of hydrodynamic radii, dependent on the length of the XTEN sequence attached, consistent with the expected bulking effects of the XTEN sequence (Fig 1B).
Alternatively, we used a three-primer genotyping protocol, where the forward microsatellite primer had an M13 sequence attached to the 5' end (5'-GTAAAACGACGGCCAG-3') and a third primer with the complementary M13 sequence was dye-labeled [68], [69].
RCA products are stretched by molecular combing, and 10 nm AuNPs are aligned along the DNA strand by hybridization of a complementary sequence attached to the particles.
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Alu repeats have been reported within loop attachment regions (LARs, sequences attached to a nucleoskeleton after encapsulation and lysis of cells under physiological conditions) [33]; however, given the prevalence of Alu repeats throughout the genome our SARs are only mildly enriched for Alu repeats (P = 0.0163, 6% enrichment for all Alu sequences; P = 0.0053, 10% enrichment for AluS repeats).
Generation of double-stranded RNAs (dsRNA) was performed as described earlier [42] using primers with the T7 primer sequences attached.
The DIG-labeled RNA probes for the sigH and rshA genes for transcript analysis were obtained by in vitro-transcription with T7 RNA polymerase, NTP-DIG-label mix (Roche Diagnostics) and gene-specific primers with a T7 promoter-sequence attached to the reverse primer (Additional file 3).
In RNA-Seq, a population of long RNA is converted to a library of cDNA fragments with adaptor sequences attached to one or both ends.
However, SPR measurements with DNA sequences attached to the chip failed because of non-specific interactions between the foldamers and the substrate of the chip.
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