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Thus together with sequence and structural analysis of YqeH, homology modeling was initiated using the monomeric transition state structure of MnmE as the template (Fig. S1, S2, S3; Fig 6a).
Protein sequence and structural analysis suggests that Dkks and colipases have the same disulfide-bonding pattern and a similar fold.
Introduction to computational biology emphasizing the fundamentals of nucleic acid and protein sequence and structural analysis, also including an introduction to the analysis of complex biological systems.
Variants were classified for functional impact based on intragenic position, existing functional data, sequence, and structural analysis.
Sequence and structural analysis showed two dissimilar residues of NbAahII10 CDR1 (Tyr27 and Tyr29) and an inserted polar residue Ser30 that appear to play an important role.
Sequence and structural analysis showed that each precursor is composed of a putative signal peptide, an N-terminal spacer, a processing site and a disulfide-bridged heptapeptide segment at the C-terminus.
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Another missense mutation in human Orc4 Y174C was also identified through sequencing, and structural analysis also predicts this mutation to be pathogenic [ 51].
Then we characterized the binding of HR1s and HR2s via both sequence alignment and structural analysis.
Multiple sequence alignment and structural analysis revealed that all members of the HSP70 family had a conserved domain architecture, with some distinguishing features unique to each HSP70.
In this study, multiple sequence alignment and structural analysis revealed that the acyl-binding residue, Gly414, of LIP1, which is located at a bend connecting α-helixes, was the non-conserved residue in five other isoenzymes.
Key amino acid residues of the salicylate 1,2-dioxygenase (SDO), an iron (II) class III ring cleaving dioxygenase from Pseudaminobacter salicylatoxidans BN12, were selected, based on amino acid sequence alignments and structural analysis of the enzyme, and modified by site-directed mutagenesis to obtain variant forms with altered catalytic properties.
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