That worship of an ideal may be his only redeeming feature at this point, but that love of meticulous sequences and precise measurements -- in the lab and in the storytelling of "Breaking Bad" -- is one of the best things about the show.
Although RIP allows identification of the target RNA molecules binding to an RBP, the data may include indirectly bound sequences, and precise locations of the binding site on the target mRNA may be difficult to determine.
This approach, which is based on 5' and 3' universal linker ligation/RT-PCR and counting of sequence reads obtained for distinct miRNAs, can in theory capture all miRNAs present in the RNA samples (including ones of novel sequence) and permits precise identification of miRNAs, even when differing by only a single nucleotide.
Annotation of ncRNAs, however, is still not part of generic annotation pipelines of genome or next-generation sequencing data and precise functional annotations of the majority of predictions remain elusive.
The minos integration site of transgenic line AsML10 was sequenced and the precise occurrence of minos-mediated integration confirmed (not shown, EMBL accession numbers AJ496323 and AJ496324).
We use the word "cocktail" even though the specific strains evaluated in our study have not been sequenced and hence precise determination of the number of FAEs in each strain is not possible.
The PCR products were sequenced and indicate precise intron integration at the desired locus.
Bioinformaticists continue to improve algorithms for detecting specific types of error, such as chimeric sequences [ 14] and precise but inaccurate reads [ 15, 16].
PNA PCR products from all samples that gave positive results by the clamped-probe assay was sequenced to confirm and precise the type of K-Ras mutation.
The UCSC Genome Browser was produced in the early stage of the human genome project and provides optical effects and precise sequence alignments on query sequences [ 1, 2].
