The 454 pyrosequencing platform, which can produce over 400 bases per read, is also superior to shorter read-length sequencers with respect to sequence accuracy for single-end sequencing.
The estimated sequence accuracy of the total repeat region was 99.8% based on a comparison with the reference sequence.
In this study, these issues have been addressed to increase signal quality and assure sequence accuracy.
The sequence accuracy and diversity of the library were validated using AmpliCot analysis (DNA hybridization kinetics) and Illumina sequencing.
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Sequence accuracy was confirmed by automatic DNA sequencing.
We calculated 454 sequence accuracy as 99.95% and variant accuracy as 99.5% excluding repeat polymorphisms.
All PCR products were cloned prior to sequencing in order to ensure sequence accuracy.
To ensure the sequence accuracy, DNA was sequenced from multiple PCR amplifications for each sample.
