Overall, the current investigation utilizing RNA-Seq will add to the knowledge of gene expression in the pig intestine.
Beyond parallel CA and GE profiling in the same single cell, scCAT-seq will be particularly useful for analyzing samples when the amount of input material is limited.
Saikia, who has a dual appointment with the veterinary college, thinks DART-seq will also help inform new approaches to cancer therapy.
Therefore, to detect these RNAs, the demand for high-throughput technologies such as RNA-seq will increase.
Our data indicate that DO-RIP-seq will be useful for quantifying RBP binding events that regulate dynamic biological processes.
3. Further investigations, such as protein interaction assessment with ATR and mapping FMRP chromatin loci with ChIP-seq, will provide deeper insight of FMRP nuclear function.
We believe that the cost and complication of analysis can improve over time, and with the advent of third generation sequencing, which has a lower rate of sequencing error (not enzyme based) and no need for amplification, the future of GRO-Seq and lncRNA-Seq will certainly be promising.
Thus, we hypothesized that the correlation between microarray data and RNA-seq data would share this dependence, since the log2-fold change in RNA-seq will have lower variance for longer genes that for shorter genes.
As a precautionary note, we cannot exclude the possibility that the OCT4-regulatory modules described here are just the tip of the iceberg and that with the adoption of an unbiased screen of OCT4 targets using ChIP-seq will reveal the complex nature of the self-renewal-gene regulatory network under the control of OCT4.
Next generation sequencing (RNA-seq) will provide a powerful tool which could be used to uncover a magnitude of more details from the complete transcriptome, including all transcripts, alternative splice isoform variants and microRNAs, to allow a deeper understanding of gene networks involved in prion disease.
For this reason, it is likely that RNA-Seq will replace microarrays in cross-species experiments.
