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Drager and Regnier have reported that optimum selectivity for some oligonucleotide separations was obtained with materials that were 40 60% quaternized.
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The best separations were obtained using monoliths modified with 15, 20, and 30 nm nanoparticles since these sizes produced the most dense coverage of pore surface with gold.
The best separations were obtained within less than 30 min employing a 10 mmol/l phosphate buffer, pH 2.8, 18 °C, 15 kV voltage.
Improved separations were obtained suggesting a favourable effect of ion-pairing interactions between analytes and the additive; however, it remained impossible to separate them all in one run.
Preparative thin-layer chromatography (TLC) separation was performed on 0.7 mm silica plates (Merck Kieselgel 60 GF254) and chromatographic separations were obtained with 70 230 mesh silica (Merck), by using n-hexane/dichloromethane mixtures.
We evaluated sample injections from buffers with varied ionic strengths and found that efficient stacking and separations were obtained in both low and high conductivity buffers, including physiological buffer with at least 140 mM salt.
Representative and reproducible 2D gel protein separations were obtained for all sludge samples.
Chromatographic separations were obtained by reverse phase LC on a 2.1 (i.d).
Chromatographic separations were obtained using a fused silica capillary column HP-5MS (30 m x 0.25 mm, Agilent Technologies).
Chromatographic separations were obtained using a fused silica capillary column HP-5MS (30 m × 0.25 mm, Agilent Technologies).
Chromatographic separations were obtained under gradient conditions using a CAPCELL PAK C18 MG analytical column (250 × 2.0 mm, 5 μm particle size; Shiseido, Japan).
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