Sentence examples for separation with a flow from inspiring English sources

In TCB, it is possible to obtain rapid size-based separation: with a flow rate of 0.7 ml/min, the run time is less than two minutes for a series of polystyrene standards.

An isocratic elution programmed with mobile phase A (96%) and mobile phase B (acetonitrile, 4%) was conducted for chromatographic separation with a flow rate of 1 mL/min.

The mobile phase which consisted of an MeOH ACN 3.3% aqueous solution of glacial acetic acid (35 35 30, v/v/v) permitted good separation with a flow rate of 0.8 ml min−1.

The 8-μL injection volume was trapped on a 0.3 × 5 mm Zorbax SB C-18 column using 3% acetonitrile (MeCN) and 0.1% formic acid at a flow rate of 20 μL/min for 16 min. A 75-μm × 50-mm Zorbax SB C-18 column, 3.5-μm particle size (Agilent Technologies), was used for analytical separation with a flow rate of 300 nL/min.

This study examines a new method of producing GC×GC separations with a flow modulator.

Peptides were first trapped and preconcentrated on a C-18 precolumn (Dionex) at 30  μL flow for 5 min and later eluted on the separation column with a flow of 220 nL/min (column dimensions were I.D. 75  μm, length 15 cm, PepMap C-18, 3  μm, 100 Å).

The PAD system in gradient HPLC mode was evaluated by analyzing the five test compounds after HPLC separation with a decreasing flow-rate gradient.

To maintain a constant concentration of MeOH after the HPLC column, a second gradient with an increasing flow-rate was included in the system, after HPLC separation, with an initial flow-rate of 700 μl/min H2O MeOH (4:1) for 4.5 min.

Separation was performed with a flow rate of 250 nl/min using a binary gradient starting at 5% solvent B rising to 60% in 50 min. Peptides were directly eluted into an ESI ion trap LCQ Deca XP Plus mass spectrometer (Thermo) with the acquisition duty cycle set to a full scan mass spectrum (m/z 400 1500) followed by two data-dependent MS/MS scans.

Separation was performed with a flow rate of 0.3 mL/min, holding at 40% B for 2 min before increasing to 100% B over 30 min. The flow was held at 100% B for 15 min prior to a return to starting conditions.

Samples were run on a highly resolving C18 column (Gemini-C18, 3 μm, 110Å, 3 mm×250 mm, Phenomenex) and peptide separation was achieved with a flow rate of 0.20 ml/min (solvent A, 0.1% trifluoroacetic acid and solvent B, acetonitrile/0.08% trifluoroacetic acid).