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Stationary phases using three different interaction mechanisms were tested to obtain sufficient separation power and separate the antiviral drugs from matrix constituents.
Furthermore, the separation power of the stepwise elution was sufficient to separate peptides with differing numbers of phosphorylated residues.
In the choice of the channel dimensions and the flow rates applied a compromise has to be found between separation power and the detectability (dilution) of the sample compounds to be separated.
One had to make a choice between settlements and separation," Power said.
The dE/dx was measured as a function of βγ to calculate the particle separation power.
In general, the RP-Amide column provided the highest separation power with different mobile phases.
Thus, ions are focused before separation, which results in improved separation power.
Based on the separation power as an alternative way to represent the performance characteristics an estimate of the next level of what might be the ultimative separation power is given.
Multi-linear gradient programs on the other hand are only scarcely used, despite their intrinsically larger separation power.
From chromatographic test measurements it was found that the separation power primarily depends on surface coverage and alkyl chain length.
The separation power achieved is thus at least 7000 (FWHM) and increases for longer time-of-flight.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com