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There was no separation possibility in the case of M15 mobile phase; tailed spots of CS and XO and double spots of TZ and AR were obtained as reported in Table 6.
A series of experiments was constructed to digest the monazite sample with a low amount of concentrated H2SO4 and to explore the separation possibility of Th from REEs in successive steps at different BaCl2 weights that act as precipitating agent.
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By adding acetone, compact spots were obtained showing improved separation possibilities.
Tailed spots in M13 of CS and non-detectability of XO and BP in M15 hampered the separation possibilities.
There are no separation possibilities in most cases due to formation of either tailed spots or double spots of dyes (Table 5).
Effects of concentration level of 1-methylimidazolium chloride as impregnant and its substitution by other ionic liquids (1,2,3-trimethylimidazoliummethyl sulphate, 1-ethyl-3-methylimidazolium tetrafluoroborate) were also studied to decide the optimum experimental conditions for better separation possibilities.
It can be conclude that the use of 1-methylimidazolium chloride in stationary phase as impregnant and 2-methyltetrahydrofuran as mobile phase provides favourable environment for better separation possibilities of biosurfactants.
In order to obtain improved separation possibilities, silica gel G was impregnated with different concentrations of ionic liquid (1-methylimidazolium chloride; 0.1~10%) and the resultant stationary phases (S2-S5) were used for chromatographic analysis of biosurfactants.
(b) 1,2,3-Trimethylimidazolium methyl sulphate(M 6 –M 10 ): In M6 and M7 mobile phases on S3 stationary phase, CS, XO and BP showed broad spots and both the BB and AR remained near the point of application; hence, no separation possibilities arise.
James Madison told them that the Constitution had a "precaution" against that possibility: separation of the government into legislative, executive and judicial branches.
Increased stability, easy catalyst separation and the possibility to reuse the enzyme in immobilized form can overcome this difference.
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