The methods most commonly used in NBS for SCD for separation of the hemoglobins in dried blood specimens are HPLC, isoelectric focusing (IEF), and CE [ 32, 35– 35].
RTM are lyophilized controls containing hemoglobins F, A, E, and S and hemoglobins F, A, D, and C, respectively, to monitor the precise separation of the aforementioned hemoglobins by the HPLC instrument [ 46].
This analyzer is not subject to interference by urea, as this reverse-phase cation exchange high-performance liquid chromatography (HPLC) method provides good separation of A1C from carbamylated hemoglobin A1.
However, there are microfluidic systems which employ the intrinsic magnetophoretic properties of hemoglobin, found in eryrthrocytes, for separation.
Nonetheless, we cannot exclude the possibility that certain peptides might have been lost due to this separation technology, but, based on our overall sequence coverage of hemoglobin, this potential loss appeared to be minimal.
The authors thank Annie Jensen for performing analysis of hemoglobin and blood cell counts and for separation of PBMC.
She later investigated the structure of hemoglobin.
Dr. Max Perutz devoted his whole career to a single problem, the structure of hemoglobin.
Dr. Waelsch later investigated the inheritance of different types of hemoglobin in mice and other mammals.
Tells of advances in the area of hemoglobin disorders, including sickle-cell anemia.
