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Dakin's solution has neither disadvantage; its solvent action on dead cells hastens the separation of dead from living tissue.
Manual classification of the point cloud allowed for the separation of dead coral framework (28% or ~162 m2) and live coral (7% or ~45 m2) (Fig. 6).
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If the cell suspension contains lower than 70%% of viable cells, a separation of the dead cells from the viable cells by density gradient centrifugation with Percoll® is highly recommended.
Prior work on DEP analysis and separation of live versus dead Gram-positive or Gram-negative bacteria has been accomplished using either ac fields (10 kHz 10 MHz) on electrode-based devices or using purely dc fields on electrode-less devices with insulating constrictions.
This device allows the separation of the initial 500 mL of dead space air from the remaining alveolar air collected in a gas-tight bag.
The infected cells were purified by centrifugation through a density gradient on Lymphocytes Separation Medium (Eurobio) to get rid of dead cells and debris, and seeded in LMI medium.
The separation of live coral colonies from dead coral framework however, was not possible and relied on accompanying video transects.
Moreover, the separation of two cells by a dead cell resulted in recovery of the stem cell reprogramming rate (Fig. 3a), indicating that an inhibitory signal is produced in one or both of the isolated cells and that the signal transmission needs a living adjacent cell.
4.3.6.3 Separation of larvae from egg shells, dead eggs, and other debris.
Lots of dead Frenchies.
"Numbers of dead".
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