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The discriminant function for separation of clusters in CD patients indeed could not clearly separate the clusters in healthy controls (Figure 2, panel C; and CDA analysis 2).
So, the separation of clusters is not assessed only by the distance of their centers.
Also, a new approach to separation of clusters is proposed and utilized in designing the objective function.
Out of the several ones the Kohonen network architecture yielding the best separation of clusters was chosen.
Also, in objective function, the separation of clusters is considered in addition to the compactness within clusters.
The partial least-squares discriminant analysis (PLS-DA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) exhibited a significant separation of clusters between the two groups (Supplementary Fig. 3B-D).
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These clusterings determine the separation of cluster A (NaCl particles) from Cluster B (mainly composed of aluminosilicate particles) and then the separation of Cluster C (Soot particles).
A VIP plot was used to identify the metabolites according to the orders of their contributions to the separation of clustering (Fig. 2c).
During one spin period, 42 cases of ESWs are continuously observed during 2 s by SC2 while other spacecrafts do not "detect" them, suggesting that the spatial scale of ESWs is limited in the separation of Cluster (smaller than 200 km, ~15 d e ).
The main conclusions are addressed as follows: (1) During one spin period, 42 cases of ESWs are continuously observed during 2 s by SC2 while other spacecrafts do not "detect" them, suggesting that the spatial scale of ESWs is limited in the separation of Cluster (smaller than 200 km, ~15 d e ). (2) The ESWs exhibit perfect symmetric structure with a small offset about 1.5 mV/m in the amplitude.
Separation of cluster compounds were conducted on an ÄKTApurifier HPLC system equipped with a Superdex 75 PC 3.2/30 (2.4 mL) size exclusion column.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com