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Ideally we would have separated the two mucins but this was not possible using current separation methodologies (data not shown).
This paper aims to review current applications incorporating different advanced separation methodologies, and their roles in achieving high resolution aroma analysis.
Each method, including different combinations of each, was insufficient to allow for chromatographic purification, was found to interfere with biochemical separation methodologies, or was found to be too harsh and damaging to protein integrity.
However, these aggregates could clearly be removed by SE-HPLC or other size-exclusion separation methodologies.
Mass Spectrometry analysis in combination with modern separation methodologies is one of the most powerful and versatile techniques used to study glycoproteome, because of its strong capabilities of providing both qualitative and quantitative information [ 8, 9].
More importantly, the interaction between ICG-sOSu and panitumumab in the SE-HPLC isolated conjugation reaction product was found to be both covalent and noncovalent in nature and would posit that the use of other size-exclusion separation methodologies would provide the same conclusion.
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Xylitol production and separation methodology by a biotechnological route using the benefits of membrane technology were investigated.
The optimized separation methodology, recently developed by the authors (Part I), was repeatedly performed on CCC columns of different design available at most research laboratories across the world.
Furthermore, the size distribution shows cross-contamination of the exosomes with small vesicles due to limitations of the separation methodology.
ICA is primarily a blind-source separation methodology and relies minimally on any a priori temporal information of the task itself [20].
In addition, we are aware of the limitations of using Ficoll density gradient cell separation methodology.
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