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CD4 T cells were separated from all mononuclear cells (PBMCs) using a magnetic separation kit (Miltenyi, USA).
DCs were isolated from 3- and 10-week granuloma preparations using a CD11c MicroBeads Cell Separation Kit from Miltenyi Biotec (Auburn, CA).
Single-cell suspensions of hind leg bone marrow from Lmna+/+ and Lmna-/ mice were T cell- depleted by staining with PE-conjugated anti-CD4 and anti-CD8 followed by removal of PE-labeled cells using the EasySep PE selection separation kit (Stem Cell Technologies; Vancouver, Canada).
Tissues were homogenized using NER nuclear separation kit (PIERCE) according to the manufacturer's instructions.
NK cells were isolated from splenocytes using the MACS® NK cell separation kit (anti-DX5 microbeads, Miltenyi Biotec).
miRNA Quantification DX5+ NK cells were isolated from whole splenocytes (MACS® NK cell separation kit, Miltenyi Biotec) and lysed in peqGOLD TriFast reagent (PEQLAB).
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Isolation of CD8+, CD4+CD25+, and CD4+CD25- cells from other mouse strains was performed using MACS microbead separation kits (Miltenyi Biotec Inc., Auburn, CA).
Murine naïve CD4+ splenocytes were isolated using a combination of magnetic separation kits (Miltenyi Biotec, Auburn, CA, USA).
QuantiGene 2.0 Plex Magnetic Separation Assay kit was used (Affymetrix).
We have 3,500 video not only for our reagent spot-testing kits, but something else we are developing called a separation-test kit, which is based on thin-layer chromatography.
Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll (Biochrom, Berlin, Germany) gradient and CD4+ cells were isolated by magnetic bead separation using STEMCELL EasySep Human CD4+ T Cell Enrichment Kit according to the manufacturer's instructions.
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