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Most metaphase NC-Rad21 cells appeared normal at this level of resolution (Fig. 5J), but unexpectedly, 28% of anaphase cells had phenotypes consistent with centromere separation in the absence of complete chromosome arm separation (Fig. 5K,L).
If this were true, artificial destruction of centrosome cohesion by C-Nap1 depletion should be sufficient to allow centrosome separation in the absence of Plk1 activity.
To compare the timing of centrosome separation in the absence or presence of Cdk1 activity in more detail, we analysed centrosome separation in cdk1as cells that were pre-synchronized in G1 by elutriation and progressed to G2/M phase in the presence or absence of Cdk1 inhibition by 1NMPP1.
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It is well known that the dynamics of this type of ordered granular media is strongly nonlinear due, (i) to Hertzian interactions between adjacent beads, and (ii) to possible bead separations in the absence of compressive forces and ensuing collisions between neighboring beads.
To obtain all the Euclidean area separations in the absence of such reliable information would thus have necessitated a number of unsubstantiated assumptions, whereas the use of border distances requires fewer constraints.
However, for the simple catalyst series studied herein, this increased lifetime comes at the expense of slower charge separation kinetics which, in the absence of hole scavengers, reduces the yield of charge separation.
The comparative experiments proved that the effective separation of Ni2+/Co2+ in the absence of EDTA was impossible.
This was achieved through adaptation of our novel reversed-phase sample displacement chromatography (SDC) method, where the major separation process takes place in the absence of organic modifier, to modular solid-phase extraction (SPE) technology.
The key feature of this model is maintenance of an inactive state by ECD-enforced separation of the TMs in the absence of ligand.
For example, major separation alone in the absence of maltreatment (e.g., in care or divorce proceedings)—can increase the likelihood of a D classification of an infant in the Strange Situation Procedure.
In Figure 3b, we present the extracted-ion chromatogram resulting from the ion mobility separation showing the absence of the matrix effect on drift time.
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