Fast separation checks are applied to place ellipsoidal and in particular arbitrary shaped particles.
After separation, we check whether the separated trajectories should be integrated by comparing the average likelihoods to avoid that the trajectories which belong to the same object are separated into different groups.
The cells were incubated with the undiluted proteolytic and collagenolytic enzyme mix for 15 minutes at 37°C and then gently dissociated by pipetting to ensure a single cell suspension; cell separation was checked by eye by light microscopy.
Achievement of equilibrium was determined by the overlap of scans at 4 h separation and checked with SedFit.
The prepared formulation was diluted 10 times with continuous media and acetate buffer and the effect of dilution on globule size, percentage transmittance and phase separation was checked.
The separation efficiencies were checked by western blot assay (Supplementary Figure S1, available at Carcinogenesis Online) and total proteins of the three fractions were separated via 2-DE in triplicate, ensuring the spot reproducibility of more than 80%.
He had access to Lynn's email account and, at the trial, computer experts showed that in the weeks after their separation he obsessively checked up on her, without her knowledge.
As a philosopher he accepted strict limitations on the faculties of the mind, and his political philosophy is moderate and sensible, aimed at a balance of power between the executive, the judiciary, and the legislature, though with a bias toward the last (see separation of powers; checks and balances).
In terms of hydrogen separation factor, the checked arrangement provided 77.75% separation which was higher than the parallel arrangement by 4.92%.
The capability of the model to represent the separation processes was checked against field tests conducted with actual fluids in full dimension prototypes.
