Sentence examples for separation cells were washed from inspiring English sources

After separation, cells were washed twice in ice cold PBS buffer containing 2 mM EDTA.

After separation, cells were washed twice in ice-cold phosphate-buffered saline (PBS) buffer containing 2 m m EDTA and the purity in selected samples was confirmed by flow cytometry.

After gradient separation the cells were washed and transferred to proliferation medium.

Cells were washed and preceded to magnetic separation.

After 15 min of incubation at 4 °C, cells were washed once and subjected to autoMACS separation for the isolation of CD4+ and CD8+ T cells.

Cells were washed in PBS and boiled in Laemmli loading buffer before SDS-PAGE separation, loading 20 μg of total cell protein per lane.

Cells were washed in PBS, homogenised and boiled in gel sample buffer before SDS-PAGE separation.

The labeled cells were washed twice and diluted in 1 ml PBS and filtrated in pre-separation filter 30 μm (Miltenyi Biotech, Lund, Sweden) before flow cytometry analysis.

Cells were washed 3 times, resuspended in 150 ml of MACS buffer and subjected to magnetic separation.

A total of 1 × 10 cells were washed with phosphate-buffered saline (PBS) and lysed on a plate with lysis and separation buffer containing a protease inhibitor cocktail.

The cells were washed with PBS, fixed, and washed again.