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PBMCs were obtained using Ficoll separation and stored in DMSO/FCS at −80°C according to previously described methods [5].
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The peripheral blood mononuclear cells were separated using lymphocyte separation medium (Ficoll-paque™-plus) and stored in liquid nitrogen until use.
One aliquot was incubated with acALY18 antibody and the other with XBP1 antibody for 30 min, then the beads were isolated by magnetic separation, washed 3 times with PBS, and stored in 100 μL PBS until needed.
After separation cells were immediately cultured or cryopreserved and stored in liquid nitrogen.
The gels were stained with 0.1% (w/v) coomassie brilliant blue R-250 (Sigma) overnight, destained and stored in 5% acetic acid at 4°C. 2D-PAGE gel separation was performed with both technical and biological replications of three.
Between each successive extraction, separation was effected by centrifugation at 10 000 rpm for 10 min. The supernatant was removed and stored in a 50 ml bottle at 4°C until analysis.
Subsequently, the mixture was centrifuged and stored in a refrigerator (−17 °C) for at least 20 min in order to obtain separation of the phases.
But in his 70th year a bitter separation dispute with his wife meant that by late summer 1939 everything on his studio walls had been taken down, crated and stored in bank cellars for lawyers to fight over.
And stored in Google's search index.
Remove and store in Ziplock freezer bags.
Wipe down and store in proper location.
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