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PBMCs obtained from patients and healthy volunteers were isolated by standard density gradient centrifugation using Ficoll (Biocoll separating solution, Greiner, Bio-One, Germany) separation and cryopreserved in 90 % FBS and 10 % DMSO.
Peripheral blood mononuclear cells (PBMCs) obtained from patients and healthy volunteers were isolated by standard density gradient centrifugation using Ficoll (Biocoll separating solution, Greiner, Bio-One, Germany) separation and cryopreserved in 90% FBS and 10% DMSO.
After incubation for 48 72 h at +4 °C, the antibody-bound peptide was separated from the free peptide by the addition of separating solution.
Then, (5) is a contrast function if and only if its maximization yields a separating solution, that is if and only if | κ 4 ( a ) | > | 3 κ 4 ( a ) + 2 4 |.
PBMC were separated by density gradient centrifugation using Biocoll (Ficoll®) separating solution (Biochrom, Berlin, Germany).
In brief, total mononuclear cells (MNCs) were isolated from blood by density gradient centrifugation with Biocoll separating solution (Biochrom AG).
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