Microscopically, this mass was composed of loosely arranged confluent undulating cords of polygonal to columnar epithelioid cells separated by a fine fibrovascular stroma resembling the placental labyrinth.
Adult worms were obtained by hepatoportal perfusion 42 days post infection and gently separated using a fine brush.
The ascospores were separated with a fine glass needle and incubated at 25°C for 24 h.
The asci were separated with a fine glass needle and, after 20 to 30 min, were gently crushed to release the ascospores.
The bundles of ovarioles were separated by a fine needle and counted under stereomicroscope [ 40]. c) Lifetime Fertility assays: The same set of vials, which were used to assess lifetime fecundity of a single female, was also used in this assessment.
In brief, after retrograde perfusion with Ca2+-free Krebs Ringer buffer (35 mM NaCl, 4.75 mM KCl, 1.19 mM KH2P04, 16 mM Na2HPO4, 134 mM sucrose, 25 mM Na2CO3, 10 mM glucose, 10 mM HEPES, pH 7.4, with NaOH) and digestion with collagenase solution (Collagenase II, 8 mg·mL−1), the left ventricular myocytes were separated using a fine scalpel and scissors.
Second, the pericarp and mesocarp were separated using fine pliers, a small sharp scalpel, or a nylon toothbrush, and subsequently rinsed until free of soft tissues.
There may be a further transition to cells separated by fine connective tissue and assuming a spindled configuration, similar to fibroblasts or Schwann cells.
Using a few separate jars is a fine idea, but if children can get into them, they might reallocate money from the Saving jar to the Spending jar.
The brain was quickly removed and the Fr2 and SS cortices were separated with fine jeweller's forceps under a binocular microscope and frozen at –80 °C until use.
