It separately regulates the expression of OXPHOS subunits, leading to a rapid modulation of mitochondrial functionality by integrating the regulation of complex IV activity with coupling efficiency and ROS detoxification processes.
For pEXP-hCRs in the five strains, three concentrations at 0.1 mM, 0.2 mM and 0.3 mM of IPTG were tested for gene expression, while five different concentrations at 0.01%, 0.02%, 0.05%, 0.1% and 0.2% of L-arabinose were tested separately for the expression of each pBAD-hCRs in Top10.
We first separately describe the expression of survivin protein in cytoplasmic and nuclear compartments of ovarian tumour cells.
Furthermore, miR-155, miR-205 and miR-375 separately regulate the expression of the SHIP and PDK1 genes, which closely correlates with Akt activation.
After co-culturing BV2 cells with hUCB-MSCs, either cell type was harvested separately and the expression of each individual cytokine was analyzed by RT-PCR.
All Met-DNAs separately induced the expression of osteopontin (Chen et al, 1997), in at least one case (C9-Met-DNA) by the removal of an inhibitory transcription factor Tcf-4 from the osteopontin promoter (El-Tanani et al, 2001).
The tubulin-GAL4 (genotype: w*; tubP-GAL4/TM3, Act-GFP JMR2, Ser1, provided by Phil Batterham, University of Melbourne) and the actin5C-GAL4 (Bloomington Drosophila Stock Center, #4414) lines were used separately to drive the expression of the UAS-Fst, both resulted in ubiquitous Fst mRNA knockdown.
To test this idea, we recorded the airflow response from the β lobe and the β' lobe separately by limiting the expression of G-CaMP to either α/β or α'/β' neurons using α/β neuron specific Gal4 line c739 [37] or α'/β' neuron specific Gal4 line g0050 [32].
Therefore, we first analyzed each cell type separately by comparing the expression of genes whose methylation was low or high in promoters or gene bodies.
The two constructs were introduced into Mycobacterium smegmatis mc155, separately, and then the expression of Mb1749c and Mb1750c in M. smegmatis pPG101 was compared to that of M. smegmatis pPG102 using real time RT-PCR.
