The twisted tapes are separately inserted from the tube wall.
Two different M1 immunodominant epitopes were separately inserted in Dd external positions without destroying the particles' dodecahedric structure.
Purified PCR-products were separately inserted into the pDONR207 vector by means of BP reaction (GATEWAY®, Invitrogen, Karlsruhe, Germany).
The amplified mouse PP2A-Aα promoter (A1) or the truncated promoter fragments (A2 to A6) were separately inserted into pGL3-basic, a background luciferase reporter gene vector at Xho I and Hind III restriction sites using standard molecular cloning techniques as described before [31].
For each of the predicted structures of each sequence, all loop subsequences are extracted and separately inserted into the tree.
Then, cat and HapII- fdh were separately inserted into the Eco47 III site of T- yodC to construct plasmids T- yodC:: cat and T- yodC:: HapII- fdh.
The six XbaI/ SphI digested gene units were then separately inserted into the SpeI/ SphI digested pTL01 (Additional file 1: Figure S2C), yielding pTL02-07, eacontaininging a single gene (Fig. 2).
With use of anterolateral entry femoral aimer (S&N), the 2.4 mm guide pins were separately inserted from the outside into each portion of the PL and AM bundle footprints behind the "ridge" and anterior to the cartilage margin.
For the EA mice, two acupuncture needles (Gauge-28, 0.5 cm) were separately inserted into each acupoint and an electrical current was provided to the needles through an electrical stimulator with parameters of 2/15 Hz at an intensity level of 1 mA (Han Acuten, WQ1002F, Beijing, China) for 30 minutes, once a day, six days a week.
The acupuncture needle was separately inserted to a depth of 4 mm at the acupoints RN1 (Huiyin), RN3 (Zhongji), BL28 (Pangguangshu), BL32 (Ciliao), RN2 (Qugu) or BL23 (Shenshu), and manually rotated right then left with a frequency of 2 Hz for 1 min.
