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To facilitate the detection of Usa1 protein, Usa1 and its mutant alleles were separately fused to Flag-epitope.
They can then be separately fused to an epigenome editing system, with one attached to the targeting system and the other to the effector.
The full-length, N-terminus truncated fragment and C-terminus truncated fragment of OsGRAS23 were separately fused into the frame with the yeast GAL4 DNA-binding domain in the vector pDEST32 by the recombination reaction (Invitrogen).
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The N terminal polypeptide without the PHD finger domain and the C terminal polypeptide containing just the PHD finger were separately fused with GST.
For this, a new approach is presented that separates unreliable image regions along depth discontinuities from reliable image regions, which are treated separately and fused to the final interpolated view.
The putative NHase is 496 amino acids long and contains the usually separately encoded subunits fused into one protein connected by a Histidin-rich stretch (Figure 2).
But as these images were acquired separately and later fused, there were problems with accurate localization [ 1].
For human hrab7A wt and hrab7A K157N, Venus and hRab7 were PCR amplified separately and then fused using SOEing PCR to generate the N-terminal Venus fusions.
To generate pFM20 [pBJ114 Δ(nfsA- H ], approximately 500 bp upstream and downstream from nfsA and nfsH, respectively, were separately amplified and fused by overlap PCR.
The Gerbera proteins were translationally fused separately to both the binding domain and the activation domain, and were combined in both directions.
Each region is treated separately and results are fused by voting.
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