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Third, we identified combined reactions encoded by the same gene sets, as these reactions cannot be separately disrupted in experiments.
Total RNA extraction was as follows: both tissues were separately disrupted in Trizol reagent (1 mL/0.1 g tissue) and 0.2 mL of chloroform was added per mL of Trizol.
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While 'competing demands' is described separately from disrupted daily structure, the two overlap to produce a situation wherein the activities women had planned for their day could become vulnerable to competing interests both internal and external to themselves.
To what extent can individual cell activities be disrupted separately and together, and still maintain an apparently normal morphology?
The peptidome peptides observed show that C3dg links to C3α'1, C3c (C3α+C3β) is disrupted, and C3β exists separately during iC3b degradation.
Cell disrupted microalgae slurry was treated separately with each individual enzyme and also, both enzymes were used together to evaluate the combined effect.
The native complex was stable, persisting throughout purification, whereas a complex formed from separately isolated Mediator and pol II was disrupted by the same purification procedures.
Cardiac extension is still observed in embryos in which Nodal or Bmp signalling has been disrupted, suggesting that heart tube extension and leftward displacement are separately regulated.
In five selected cases, ER-negative cells overlying the disrupted myoepithelial cell layer and adjacent ER-positive cells within the same duct were separately microdissected and assessed for loss of heterozygosity and microsatellite instability.
The pellets were separately re-suspended in 20 mmol/L Tris-HCl pH 8.0, 500 mmol/L NaCl and 5 mmol/L DTT and subsequently disrupted by sonication.
Disrupt or be disrupted.
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