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Two cultures were separately cultivated in this investigation, involving culture LHTO4, cultivated with freshwater and culture HHTO4, cultivated with 3.5% (w/v) NaCl solution.
To evaluate GABA production, strains GAD, and GAD∆pknG were separately cultivated in GP2 medium containing 100 g L−1 of glucose using baffled flasks (Figure 3).
In 2007, the population and its parents were separately cultivated at Rayong province, Thailand.
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Lignocellulose often occurs as garden waste or as residue in various branches of industry, so these sugars do not compete with the food or feed industry and do not have to be cultivated separately like corn or sugarcane.
The LSJ1 and N2 lines were cultivated separately from shortly after the Bristol strain was isolated until methods to freeze C. elegans were developed.
Here we determine the genome sequence of LSJ1(Bristol), a strain derived from the same wild isolate as N2 but cultivated separately from it at least since 1964.
Both the cells were cultivated separately in repetitive batch mode for enhancing the biomass productivity and enzyme activity.
For the evaluation of AP activity, the osteoblasts of each group were cultivated separately in 24-well plates.
thiooxidans A01 were first cultivated separately in medium without arsenite until the middle logarithmic phase and then inoculated to 100 mL medium containing 100 mM As III), respectively.
Approximately 20 colonies with positive transformants were selected for each sample, and each transformant was cultivated separately in LB broth at 37°C for 12 h.
CD4 and CD8 cells were purified by CD4 enrichment kit (StemCell Technologies, USA) and cultivated separately to generate either Th1 or Tc1 cells as previously described [ 26].
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