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As a control, cells were treated in a separated tube with phycoerythrin-labelled mouse IgG antibody (Sigma).
In a separated tube, 0.2 mg of miRNA was dissolved in 0.6 mL of 20 mM citric acid at pH 4.0.
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Target cDNAs were amplified in separated tubes using the following procedure: 10 min at 95°C and then 40 cycles of denaturation at 95°C for 15 s and annealing and extension at 62°C for 1 min per cycle.
All participants had blood collected from a forearm vein in serum-separated tubes (without using an anticoagulant).
(B) Compared to wildtype embryos, Mesp1 embryos had two separated heart tubes on either side of the mid-line.
Blood for complement analysis was taken into EDTA tubes, separated via centrifugation (2000 g) within 4 h of collection, and stored at −70°C prior to use.
Tissues were placed in separated sterile 50-mL tubes with ice-cold culture medium and then transferred to a cell culture laboratory on ice.
All separated sera and EDTA tubes were stored at −80°C.
Blood was collected in sodium heparin tubes and separated for plasma and peripheral blood mononuclear cells (PBMCs) over Ficoll-Paque (GE Healthcare) according to the manufacturer's instructions within 18 hr of collection.
In order to explore the mechanisms of enhancement in energy absorption, the composite skeleton and the tube filled with separated foams were tested, and the strong interaction between foams and the skeleton has been found to have a very remarkable contribution to total energy absorption.
Mineral and organic tube components were separated based on grayscale values that represent X-ray attenuation, which closely corresponds to material density.
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