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separated solution method.
The separated solution carried out in this photo-reactor system can avoid CO2 reduction products being oxidized by anode.
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PBMCs obtained from patients and healthy volunteers were isolated by standard density gradient centrifugation using Ficoll (Biocoll separating solution, Greiner, Bio-One, Germany) separation and cryopreserved in 90 % FBS and 10 % DMSO.
Using a proposed heuristic procedure, we separate solution space into different parts and each subpopulation represents a separate part.
After incubation for 48 72 h at +4 °C, the antibody-bound peptide was separated from the free peptide by the addition of separating solution.
PBMC were separated by density gradient centrifugation using Biocoll (Ficoll®) separating solution (Biochrom, Berlin, Germany).
In brief, total mononuclear cells (MNCs) were isolated from blood by density gradient centrifugation with Biocoll separating solution (Biochrom AG).
MNC were isolated by density gradient centrifugation with Biocoll separating solution (Cambrex; 800×g, 20 min, without brake) from fresh peripheral blood of healthy human volunteers or patients with type 2 diabetes as previously described [11].
Afterwards, the cells were centrifuged with easycoll separating solution (Biochrom) to discard dead cells.
Mononuclear cells were isolated by Ficoll gradient centrifugation (Biocoll separating solution, Biochrom KG, Berlin, Germany).
The bone marrow was centrifuged and cells were fractionated on a density gradient (Biocoll separating solution; Biochrom, Berlin, Germany).
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