The general procedure is illustrated in a workbench fashion on a separated plastic tibia in Additional file 2: Video S2.
The mice were randomly distributed into three groups of 55 mice and housed in separated plastic cages in controlled animal house facilities for one week to acclimate their environment.
Male and female offspring were kept in separated 1L plastic boxes, three-quarters filled with moist sand, and topped with ad libitum fresh homogenised dung.
Blood samples were centrifuged at 2000 ×g for 15 min, and the separated plasma was transferred to plastic tubes.
The separated plasma was collected using a plastic pastette and transferred to clean tubes for immediate storage at −80 °C until assayed using radioimmuno assay with commercially available kits according to the manufacturer's instructions (EURIA-CCK, Euro-Diagnostica, obtained from Immunodiagnostic Systems, Ids Ltd., Tyne and Wear, UK) as described previously.
After collection, the experimental animals were individually marked on the thorax with enamel paint and separated, according to sex, into two rectangular plastic containers (40×50 cm).
The plastic wrap keeps everything clean and separated.
The plastic flow of matrix and the void growth are dealt with separately after we show that two separated loading sub-surfaces and corresponding normality rules for matrix and damage exist.
For this test, an OF system made of gray plastic (50×50×40 cm [height]) with four equally separated holes (3 cm diameter, each with an infrared sensor) on the floor was used (model ST-1/WII, Muromachi-kikai, Tokyo, Japan).
Olfaction was measured in a separated 3 min test: the mouse was placed in a plastic transparent cage [17 cm (d) × 28 cm (w) × 17 cm (h)] with a drop of diluted (1 100) almond oil (Langdale, England) on the wall 4 cm from the floor.
