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We composited the samples, separated plant material by species, and separated green vegetation from standing dead tissue and litter.
The separated plant samples were oven dried at 70 °C for 48 h and then grinded with an aid of a mortar and a pestle to achieve homogeneity of the samples.
This suggests that these miRNAs share common features with all the widely separated plant lineages recorded in the database miRBase.
The chromatograms were air dried and sprayed with 0.2%% 2, 2-diphenyl-2-picryl-hyrazyl (DPPH) (Sigma®) to detect any antioxidant compounds present in the separated plant extracts.
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This means, they believe, that someone would have to have poisoned two different batches of pills at two widely separated plants with the same batch of poison.
Are there any samples (rbcL sequences) from geographically well separated plants from the same species?
Care was taken to collect seeds from spatially separated plants (at least 1 m apart) to avoid resampling individuals.
Work began on a reactor at Marcoule in the summer of 1954 and on a plutonium separating plant the following year.
Biological replicates were generated by separating plants into two groups.
Weeded plants were identified, counted, separated into plant functional groups (grasses, herbs and legumes), dried (60°C, 72 h) and weighed.
After you have separated the plant into parts, inspect each part you are preparing for parasites.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com