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After the AppSeed prototype stage is done and dusted, designs can then be handed off for the next stage of the design process by being sent to Photoshop as a layered file (i.e. with each button or element separated onto its own layer for easier manipulation).
About 200 µg of the total pooled RNAs were separated onto a denaturing 15% polyacrylamide gel.
Total proteins were separated onto SDS-PAGE gels, which were dried and exposed to X-ray films.
After 41 h incubation the trophozoite-enriched fraction was separated onto and collected from a discontinuous 10/40/80% Percoll gradient.
Fifty micrograms of total cellular protein were separated onto 12% Nupage gels Tris-HCl sodium dodecyl sulfate (SDS -PAGE, tranSDS -PAGEntransferredlontoVDF membranes, and blocked with 5% natural nonfat dry milk as described before.
Equal amounts of total proteins (40 µg) determined by the Bradford method (BioRad, Marnes-La-Coquette, France) were separated onto 12% SDS-PAGE then transferred on nitrocellulose membrane (Schleicher & Schuell, Dassel, Germany).
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Before you get too excited: 3.0.1 and 3.2 are likely near identical copies of 3.0 at this point, just separated out onto individual branches for the sake of developing them in parallel.
Western blotting: Transfer PAGE separated proteins onto nitrocellulose membrane using Western blotting semi-dry transfer apparatus.
Similarly, we also separated males onto plates as L4s from each of the seven experimental strains (AB1, CB4855, CB4856, DR1350, JU440, MY2) and the reference strain (PD4790).
Sprinkle the separated petals onto the sugar in the jar.
Protein (50 μg) was loaded onto 12% SDS PAGE gels, separated, and transferred onto nitrocellulose membranes (Bio-Rad).
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