Sentence examples for separated nuclei from inspiring English sources

On the other hand, nonsolvent with a large viscosity could prevent the growth of the phase separated nuclei, leading to the presence of relatively small and discontinuous pores on the microsphere surface, which can finally cause the decrease of the contact angles.

Samples of the pellet fractions were subjected to immunoprecipitation. Second we separated nuclei from the other cell fractions as described previously [47].

After an initial stage during which the cells contain well separated nuclei with intact envelopes, nuclear fusion (karyogamy) occurs.

Apoptotic cells were identified by condensation and fragmentation of the nuclei (separated nuclei composed by clusters of blue dots).

In these algorithms, only the separated nuclei are chosen automatically by the ACIS or manually chosen by the operator; nuclei that touch each other are recognized by the ACIS through their size and other morphometric parameters and are separated by insertion of a single pixel-wide boundary at the point of contact.

The reason is that fluorescently labeled DNA will remain in the excitation focus for a longer period of time when there are two separated nuclei than when there is only one nucleus in the cell.

After separating nuclei, unlysed cells and glass bead, samples were loaded and run on a 4 20% Precise gel (Pierce).

After addition of Tritox X-100 and vortexing, 1.5 mL of each sample was aliquoted into an eppendorf and centrifuged to separate nuclei.

The cytoplasmic proteins were separated from nuclei by down-centrifuging the nuclei for 5 minutes at 2300 g and were subsequently ethanol precipitated.

The cytosolic fraction was separated from nuclei by 7 min centrifugation at 4°C at 9000 rpm.

Protein extracts were separated from nuclei by centrifugation at 3000× g for 10 min at 4 °C and quantified by performing a Bradford assay (Bradford Reagent, SIGMA).